What Is Touchdown PCR?

Touchdown PCR (Polymerase Chain Reaction) is a technique used to amplify DNA fragments from a complex mixture of DNA.

The method involves gradually reducing the annealing temperature during the PCR reaction, which aims to increase the specificity of the amplicons and reduce the formation of non-specific products.

The touchdown PCR technique helps in minimizing the formation of unspecific amplification products by allowing the annealing of primers to occur under stringent conditions in the initial cycles of PCR, making it a useful method for amplifying genomic DNA with high sensitivity and specificity.