PCR plates are commonly used in molecular biology to amplify DNA sequences. The sealing of PCR plates is crucial to prevent evaporation or contamination during the PCR process. Here are some methods for sealing PCR plates.
1. Adhesive seals: Adhesive seals are pre-cut adhesive films that are designed to cover the wells of the PCR plate. They are easy to apply and remove and provide a tight seal to prevent contamination. Adhesive seals are available in different materials including polypropylene, polyester, and aluminum.
2. Heat seals: Heat seals are applied by using an automated heat-sealing machine. The machine heats up the plastic film to create a strong seal over the PCR plate wells. Heat seals are more secure than adhesive seals because they can withstand higher temperatures.
3. Cap strips: Cap strips are used to cover the wells of the PCR plate. They are made of polypropylene and have 8 or 12 caps that are snapped onto the PCR plate. Cap strips provide a secure seal and are easy to remove.
4. Sealing mats: Sealing mats are designed for use with 96 well plates and PCR plates. They are pressed onto the plate to provide a tight seal and prevent contamination. Sealing mats can be reused after sterilized.
In conclusion, there are different methods available depending on the type of PCR plate used. Adhesive seals, heat seals, cap strips, and sealing mats are all effective ways to seal PCR plates. By choosing the right sealing method, researchers can ensure the accuracy and reliability of their PCR results.