Elisa, short for Enzyme-Linked Immunosorbent Assay, is a versatile plate used in laboratories for various scientific tests. Composed of 96 or 384 wells, it enables researchers to detect and quantify proteins, antibodies, hormones, and antigens in biological samples. Its design allows for precise and high-throughput analysis, utilizing specific antibodies to capture target molecules, making it pivotal in diagnosing diseases like HIV, COVID-19, and autoimmune disorders.
Elisa plates offer sensitivity, accuracy, and reproducibility, aiding in pharmaceutical research, medical diagnostics, and diverse scientific investigations, revolutionizing the way we study and understand biological processes and diseases.
Yikang Med supplies high quality 96 well Elisa Plates for laboratory research. Detachable & undetachable liquid handling consumables are available.
Elisa plates, or Enzyme-Linked Immunosorbent Assay plates, are specialized tools extensively used in biomedical research and diagnostics. These plates serve as a platform to detect and quantify biological substances like proteins, antibodies, hormones, and antigens in samples. The process involves coating the wells with specific capture antibodies that bind to the target molecules. Subsequent steps introduce detection antibodies and enzymes, producing a measurable signal when the target is present. Elisa plates are fundamental in diagnosing diseases, monitoring biomarkers, and assessing drug efficacy due to their sensitivity, precision, and ability to handle numerous samples simultaneously. They've revolutionized medical diagnostics, aiding in identifying conditions such as HIV, COVID-19, and various autoimmune disorders, while also being pivotal in diverse scientific studies and pharmaceutical research.
An Elisa plate operates on the principle of specific antibody-antigen interactions to detect and quantify substances like proteins, antibodies, hormones, or antigens in biological samples.
Here's a step-by-step breakdown:
Coating
The plate wells are coated with a capture antibody specific to the target molecule of interest.
Binding
The sample containing the target molecule is added to the wells. If the target is present, it binds to the capture antibody, adhering to the plate.
Washing
Unbound substances are washed away, leaving only the bound target molecules.
Detection
A detection antibody, also specific to the target, is added. This antibody is linked to an enzyme.
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