How To Measure The RNA Purity?

RNA purity can be measured by the absorbance ratio of the sample at 260 nm and 280 nm using a spectrophotometer. The ratio of absorbance at 260 nm and 280 nm provides an estimate of the level of contamination by proteins, nucleotides, and other impurities.

A high-quality RNA sample typically has a 260/280 nm absorbance ratio between 1.8 and 2.1. RNA samples with a 260/280 ratio outside of this range may indicate contamination, degradation, or poor extraction.

Additionally, an RNA sample can also be checked for purity using gel electrophoresis, which separates the RNA by size and visualizes the bands on an agarose gel. High-quality RNA samples show sharp, distinct bands, whereas samples with impurities may show a smear or haze, indicating degradation or contamination.