What is the difference between 96 and 384 well PCR plate?

96 and 384 well plates are both commonly used in scientific research for high-throughput screening and assays. While they have some similarities, there are also significant differences between the two. One major difference is the number of wells. The 384 well plate can accommodate four times more samples as the 96 well plate. Another difference is the well volume. Generally, the wells in a 384 well plate are smaller than those in a 96 well plate. The standard well volume for a 96 well plate is around 300 μl, while for a 384 well plate it is around 40 μl. This means that if you require less sample volume, a 384 well plate can help you conserve your reagents. The smaller well volume also means that a 384 well plate can offer higher sensitivity and precision compared to a 96 well plate. This is especially important for experiments that require small amounts of precious or rare substances. Another advantage of using 384 well plates is that they can offer higher throughput, as the number of samples that can be processed at once is much greater. However, the smaller well volume can also make it more difficult to handle and pipette liquid samples accurately. In summary, while both 96 and 384 well plates have their advantages and disadvantages, the choice will depend on the specific needs of the experiment. If one requires handling larger volumes, a 96 well plate is ideal, but if one requires higher throughput or needs to conserve reagents, a 384 well plate is the better choice.

What is the minimum volume in 384 well?

The minimum volume in a 384 well is determined by several factors, including the size and depth of the well as well as the equipment being used for liquid handling. In general, the minimum volume for a 384 well is typically around 5-10 microliters. It is important to note that when working with small volumes, accuracy and precision become critical factors. Therefore, it is essential to use high-quality pipettes and calibrate them regularly to ensure accurate and consistent liquid handling.

384-Well PCR Plates - Zhejiang Yikang Medical Technology Co., Ltd.

384-Well PCR Plates

A 384-well PCR plate is a high-density sample plate designed to accommodate 384 separate PCR reactions in a single plate. With its increased well density, it allows for more samples to be processed at once, enhancing efficiency and cost-effectiveness in laboratories. The plate is made of high-quality virgin polypropylene, ensuring a consistent and reliable plate performance. It is compatible with most thermal cyclers, making it a versatile tool for PCR amplifications. Overall, the 384-well PCR plate is an essential tool for high-throughput diagnostics and research applications, enabling faster and more accurate results.

Types of 384-Well PCR Plates

384 Well PCR Plate

YikangMed, a leading provider of laboratory consumables and equipment, is proud to introduce its latest product offering: the 384 well PCR plates. These plates are designed to provide high-throughput ...

384-Well PCR Plates Gallery

What Is the Volume of the 384 Well Plate for PCR?

The Standard 384-well PCR plate is compatible with the majority of 384 block PCR and sequencing instruments. It has a maximum well volume of 40 µL and a working volume of 25 µL.

The total volume of the 384 well plate for qPCR is determined by multiplying the volume of each individual well by the number of wells. This gives a total volume of between 1920 and 7680 microliters, or 1.92 to 7.68 milliliters.

384-Well PCR Plates FAQs

What is the difference between 96 and 384 well PCR plate?

96 and 384 well plates are both commonly used in scientific research for high-throughput screening and assays. While they have some similarities, there are also significant differences between the two.

One major difference is the number of wells. The 384 well plate can accommodate four times more samples as the 96 well plate.

Another difference is the well volume. Generally, the wells in a 384 well plate are smaller than those in a 96 well plate. The standard well volume for a 96 well plate is around 300 μl, while for a 384 well plate it is around 40 μl. This means that if you require less sample volume, a 384 well plate can help you conserve your reagents.

The smaller well volume also means that a 384 well plate can offer higher sensitivity and precision compared to a 96 well plate. This is especially important for experiments that require small amounts of precious or rare substances.

Another advantage of using 384 well plates is that they can offer higher throughput, as the number of samples that can be processed at once is much greater. However, the smaller well volume can also make it more difficult to handle and pipette liquid samples accurately.

In summary, while both 96 and 384 well plates have their advantages and disadvantages, the choice will depend on the specific needs of the experiment. If one requires handling larger volumes, a 96 well plate is ideal, but if one requires higher throughput or needs to conserve reagents, a 384 well plate is the better choice.
What is the minimum volume in 384 well?

The minimum volume in a 384 well is determined by several factors, including the size and depth of the well as well as the equipment being used for liquid handling. In general, the minimum volume for a 384 well is typically around 5-10 microliters.

It is important to note that when working with small volumes, accuracy and precision become critical factors. Therefore, it is essential to use high-quality pipettes and calibrate them regularly to ensure accurate and consistent liquid handling.